TrailBio^® Vascular Leptomeningeal Cells

Flow cytometry data shows homogeneity of TrailBio® Vascular Leptomeningeal Cells upon thawing (day 0) and post-thaw (day 7). More than 80% of cells express CD44/CD146/NG2, and 50% of cells express PDGFRA, confirming the cellular identity of neuroectoderm-derived VLMCs.​

qRT-PCR data shows high expression of selected VLMC genes, including DCN, SPP1 and COL1A1 relative to control gene GAPDH on day 7 post-thaw. In contrast, neuronal control gene TUBB3 is shows low expression in day 7 post-thaw cultures.

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Bulk RNA-seq analysis of TrailBio® Vascular Leptomeningeal Cells shows distinct gene expression signatures of VLMCs compared to iPSCs and selected relevant cell types upon thawing (day 0) and post-thaw culture (day 7).​

Protein expression of selected VLMC markers in TrailBio® Vascular Leptomeningeal Cells were validated by immunocytochemistry in post-thaw cultures (day 7). ECM proteins lumican (LUM) and collagen-1a (Col1A1) and structural protein vimentin (VIM) (expressed in endothelial cells and glial cells) were detected in the majority of cells. Absence of the astrocytic marker glial fibrillary acidic protein (GFAP) confirmed that, despite the presence of common markers between VLMCs and astrocytes, cells do not differentiate into astrocytes in culture. Nuclei stained with DAPI. Imaging performed at 20X magnification.